The EKKO™ CD microplate reader is the first well plate reader designed for circular dichroism measurements. It introduces a new fast and economic tool to meet today’s advanced pharmacopeia requirements. The EKKO™ operates like other well plate readers already on the market for absorbance and fluorescence. By eliminating the need to transfer samples to a cuvette, users significantly reduce the amount of time spent running large numbers of samples. The EKKO™ reads 96 well plates standard, and can accommodate 384 well plates as an option. The EKKO™can read 96 wells in less than two minutes. Thousands of measurements are possible per day, making circular dichroism a new high throughput and cost saving technique.
The EKKO™ also saves lab space with a footprint one fourth of conventional CD instruments with autosamplers.
Enantiomeric Excess (ee) measurements
The EKKO™ fills the need for high throughput screening in asymmetric synthesis to get enantiomeric excess values (ee) rapidly. Parallel synthesis is performed in the microplate. Enantiomeric excess can then be determined directly in the microplate without transferring solutions to another system. It offers an effective alternative to HPLC with no need to separate enantiomers, and by avoiding elution of solvent. Ten to one hundred times higher throughput is reasonable to expect from EKKO™ compared to alternative instruments and conventional CD instruments. It is the perfect tool to find and qualify enantiomerically enriched compounds rapidly.
EKKO™ can measure spectra from far UV, enabling high throughput screening of protein structures, and development of biotherapeutics. The EKKO™ is also suited for late stage research and QA/QC labs requiring high throughput.
Contact SpectroLogiX today to learn more about the EKKO and to request a quotation
Enantiometic excess (ee) values for asymmetric synthesis catalysis
High Order structure determination
CD High Throughput Screening (185-880 nm)
96 well plate reading < 2 minutes
Thousands of ee values per hour
Noise floor : as low as 0.05 mdeg (1s)
Extra low operational costs
¼ footprint of a conventional CD with auto-sampler attachment
10 to 100 x faster than HPLC or conventional CD
Detection mode Circular Dichroism and absorbance
Measurement mode Single wavelength or Spectrum
Microplate format 96 or 384 well plates
Reading Time <2 minutes (96 wells, single wavelength mode)
Minimum volume 45 ul (96 well plate format)
Wavelength range 185 - 880nm
Monochromator Dual gratings
Wavelength accuracy +/-0.1nm
Stray light 5ppm (200nm)
rms noise +/-0.08 mdeg (200nm, 8s integration time)
+/-0.02 mdeg (500nm, 8s integration time)
CD range +/-1000 mdeg
Absorbance precision 0.01 Au
Light source lifetime >9000h typical
N2 purge 0.5 l/min
Temperature Room temperature